Catalase Lab

Nevertheless, the effects caused by the breakage of bonds will eventually lead to a decrease in the rate of reaction. As seen in the data, the reaction rate increased from 0.088 to 0.101 throughout the interval of -5℃ to 20℃ then decreased to 0.037 throughout the interval 20℃ to 56℃. This can be explained by the fact that 20℃ is the optimal temperature, therefore the active site of the enzyme is complementary to the substrate, causing the rate of reaction to be

Introduction Alka-Seltzer has been on the market since 1931 and has helped to relieve indigestion and upset stomach. The tablets began to fizz and bubble when dropped into water. “The fizziness happens when baking soda (sodium bicarbonate) and citric acid react chemically in water. They yield sodium citrate, water and carbon dioxide gas, which causes bubbles.” Based on this information, we will measure the reaction time of AlKa- Seltzer dissolved in 200 ml of water at 3 different tempertures in the first portion of this experiment.

Factors that affect chemical reactions Intro: this experiment is to show us what factors affect the rate of a chemical reaction. Supplies: The Works Liquid Toilet Bowl Cleaner, TUMS antiacid tablets, a non-serrated knife, a spoon, a stirring rod, four test tubes, flame heater, a small beaker for the boiled water, rubber gloves, and safety goggles. Procedure: we filled a beaker 3/4 of the way full with tap water and brought the water to a boil. We then labeled our test tubes 1,2,3,4.

ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. Using different pH levels, the absorbance rate of the reaction was measured to see at which condition the enzyme worked best. The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds.

Catalase works by the following mechanism : 2 H2O2 ------------------> 2 H2O+ O2 Hypothesis: The hypothesis for this experiment is that the foam of O2 produced from the reaction between hydrogen peroxide and catalase will increase in height when the concentration of hydrogen peroxide increases. Independent variable: The variable that was changed during the experiment was hydrogen peroxide (H2O2).

There are few vegetables and fruits that turns to the color brown if their surface is exposed to oxygen. Once the veggies or fruits been exposed to oxygen, then the browning begins to appear, and electrons and hydrogen will be removed. This happens because of an enzyme called catechol oxidase. The enzyme will act on its substrate catechol to form a yellow compound which then reacts with the oxygen in the air and change into benzoquinone. The more concentration of the enzyme, the more browning appears.

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

Conclusion: Based on the results of molarity from Trials 1, 2, and 3, it is concluded that our experimental for each trial is .410M NaOH, .410M NaOH, and .450M NaOH. The actual molarity of the NaOH concentration used was found to be 1.5M NaOH. The percent error of the results resulted in 72%. The large error may have occurred due to over titration of the NaOH, as the color of the solution in the flask was a darker pink in comparison for the needed faint pink. Discussion of Theory:

The Rational for this experiment is, ‘Thousand cellular respiration’. The Aim of this Practical investigation is to “see what factors affect the decomposition of hydrogen peroxide by the enzyme catalase which is found in the

7. In this experiment, if the sucrose concentration were increased to 70 g/l would you expect sucrase activity to be significantly higher than the activity at 35 g/l. Explain your answer. No, because based on the results once it reached 30 g/l 35 g/l the results had stayed the same. There, the activity is lessening and coming to what looks like a plateau. 8.

Instead, with the evidence collected from the lab, it was concluded that adding hydrochloric acid to a mixture of hydrogen peroxide and liver extract hindered the original reaction and decreased bubbling. The positive control, that hydrogen peroxide and the catalase in liver extract are known to react, created more oxygen bubbles than the liver extract and acid did when combined with hydrogen peroxide. On the other hand, the negative control produced no reaction, since water does not react with hydrogen peroxide. There were a inconsistencies in data in the positive control since the first test was 8 mm higher than average and the second test was 7 mm less than average, and the tested experiment since the first test results were 10 mm less than the second and third. This might have been caused by imprecise and slow measurement or confusion due an overload of running tests.

Introduction: Enzymes are biological catalysts that increase the rate of a reaction without being chemically changed. Enzymes are globular proteins that contain an active site. A specific substrate binds to the active site of the enzyme chemically and structurally (4). Enzymes also increase the rate of a reaction by decreasing the activation energy for that reaction which is the minimum energy required for the reaction to take place (3). Multiple factors affect the activity of an enzyme (1).

Introduction In class, a series of experiments were performed that pertained to the enzyme known as catalase, which converts hydrogen peroxide into oxygen. Due to peroxide being toxic to the tissues of both plants and animals, both possess the enzyme catalase, which breaks into two non-toxic compounds: water and oxygen gas. Enzymes are proteins that react to certain substrates to create a product, and continue doing so afterwards. Methods and Materials To test reactions between catalase and hydrogen peroxide, groups of three to four people were formed.

Based on the results from Part A, the enzyme concentration is directly proportional to the rate of reaction. This means that as enzyme concentration increases so does that rate of reaction when the catalase is placed in the 140ml of 3% hydrogen peroxide. Referring back to Graph 1.1 it is evident that the there is a steady increase in rate of reaction as the concentration went up which explains why the line of best fit is positive. This relation happens because the substrate concentration of the 3% hydrogen peroxide is always in excess in comparison to the enzyme catalase concentration then as we increase the concentration of catalase there will always be a substrate that may be catalyzed. Factors that may affect this relationship would be temperature

They can only quicken reactions that will eventually occur, but this enables the cell to have a productive metabolism, routing chemicals through metabolic pathways. Enzymes are very specific for the reactions they catalyze; they make sure the chemical processes go in the cell at any given time. Peroxidase was the enzyme being testing in this experiment. A peroxidase is an enzyme that acts as catalysts, which occurs in biological systems. Peroxidase is found in plants, which they play a role in helping to minimize damage caused by stress factors or insect pests.