Procedure:
Before transfer to the experimental chamber
1. Set up the chiller 1 at 10oC and chiller 2 at 12oC.
2. Maintain the temperature of perfusate reservoirs, perfusate lines, and saline bath are at 10oC.
3. Turn on the circulation valves for chiller 1 and turn on the bypass valves for chiller 2
4. Set up the inline perfusate delivery at the constant pressure of 0 cmH2O and outflow line at 30 cmH2O.
5. Ensure that perfusate is flowing through the lines adequately
6. Clamp off the outflow line
Transfer
7. Transfer and fully immersed the fish in the saline bath of constant temperature 10oC
8. Connect the input cannula immediately to the line delivering perfusate while completely immersed in water
9. Connect the output cannula to the outflow line while completely immersed in water
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Turn off the circulation for chiller 1 and turn on the circulation for chiller 2, and start sampling
11. Increase the temperature of chiller 2 to 12.5oC and keep it at the same temperature for 6 minutes OR alternatively, raise the initial chiller 2 temperature at a faster rate.
12. Keep raising the temperature at the rate of 0.5oC every 6 minutes until arrhythmia is reached and determine TArr.
13. As TArr is approached, stop and bring the temperature of the heart down to 5oC lower than TArr. (e.g. if TArr 25 oC then temperature of the heart should be 20 oC) (switching chillers here )
14. Following step 12 determine if the same TArr is reached. Repeat this step acceptable number of times.
• If TArr is not the same after repetitions, then follow the single intervention procedure.
• However, If TArr is same after repetitions, then follow the multiple intervention procedures.
Single intervention procedure:
15. Use cholinergic agonists to slow down the heart rate and determine TArr for each case:
a. Inject Carbachol after step
One way we could improve the experiment is by doing more trials, the more trials the more accurate the resolutes are. Another way we could improve the experiment is to have more time so we could make sure all the temperatures
Next, about 10 mL of both solutions, Red 40 and Blue 1, were added to a small beaker. The concentration of the stock solution were recorded, 52.1 ppm for Red 40 and 16.6 ppm for Blue 1. Then, using the volumetric pipette, 5 mL of each solution was transferred into a 10 mL volumetric flask, labelled either R1 or B1. Deionized water was added into the flask using a pipette until the solution level reached a line which indicated 10 mL. A cap for the flask was inserted and the flask was invented a few times to completely mix the solution. Then, the volumetric pipette was rinsed with fresh deionized water and
The temperature probe was then quickly cooled to room temperature. When this was achieved, the hot water was immediately transferred into the calorimeter. This method of keeping the temperature probe cooled before measuring a new temperature was repeated throughout the entire experiment. Temperature data was collected for 180 s while swirling the temperature inside the calorimeter. The calorimeter still contained the warm water.
After the data is collected, a graph of water temperature and amount of blubber will be
These difficulties might have caused me to make mistakes on the amount needed to pipette or the type of substance. Overall, next time I would have made sure to pay closer attention to the pipetting portion of the procedure. Furthermore, we could extend this experiment by trying different kinds of
Next, this test was repeated for tubes C-E. Following the completion of the tests, all test tubes, pipets, and well plates were cleaned out and liquid waste was disposed of in the proper container. Once all data was collected, results were shared in class. Following this, all previous steps were repeated for the second and third stage. Following the end of all 3 stages.
As mentioned in the hypothesis, the prediction is that as the temperature increases towards the optimal, the rate of respiration will increase. As the temperature exceeds the optimal, the rate of respiration will decrease. The temperature of the environment can be varied by placing the respiration chamber under a temperature-controlled water bath/cooling bath. The temperatures that will be used in this experiment will range from 0ºC to 50ºC in 10ºC increments. Digital thermometer will be used to measure the temperature of air.
Phase II(sub-acute phase): When you leave the healing facility, your heart recovery project will proceed at an outpatient office. Stage two of cardiovascular restoration more often than not endures from 3-6 weeks and includes kept checking of your heart reactions to practice and movement. Another imperative part of stage two cardiovascular restoration is training about fitting activity methodology, and about how to self-screen heart rate and effort levels amid activity.
compress the chest to a depth of 1 1/2 - 2 inches with the number of compressions to breaths 15:2 this is for adults. Do not rock back and forth or sit on your heels while performing compressions, blood will not be pumped out of the heart. Roll your hands on the victims chest, do not lift your hands off the victims chest or bounce your hands on the
Modifications of this procedure include the use of hot plates instead of Bunsen burners, and heating t-butyl alcohol to 60-65 ℃ instead of 50 ℃. Other modifications include the use of weighing boats to measure an amount of unknown instead of weighing paper, and completing one run of unknown 2 instead of two runs of unknown 2. Summary of
Materials: The materials that I will be utilizing during these experimentations are three to four ice cubes, one cup for measuring, six unblemished cups, one stopwatch, one hot water source, three tablets of Alka-Seltzer, one thermometer that measures from negative
\section{Facility Static and Dynamic Control}\label{Calibr} The facility calibration is the transfer function between the oscillating gauge pressure $P_C(t)$ in the chamber (described in ~\autoref{Sub31}) and the liquid flow rate $q(t)$ in the distributing channel, i.e. the test section. Due to practical difficulties in measuring $q(t)$ within the thin channel, and being the flow laminar, this transfer function was derived analytically and validated numerically as reported in ~\autoref{Sub32} and ~\autoref{Sub33}. \subsection{Pressure Chamber Response}\label{Sub31} Fig.\ref{fig:2a} shows three example of pressure signals $P_C(t)$, measured in the pneumatic chamber.
5. Pressing the heart massage to massage the rhythm. In the speed 100 times / minute, if less than this will not work. I hope that this how to do CPR. will be benefit with you or someoneyou help.
Rediet Legese iLab Week # 6 CRUDE OIL DISTILLATION Introduction: The aim of this week lab experiment is to experiment distill crude oil and to check how temperature determine the chemical properties of crude oil plus how the boiling point can also show physical properties. They are two major finding in this experiment. he first finding was the point at which the raw petroleum is heated to the point of boiling, at 275 0C, the gas and kerosene oil are refined, however the oil (lubricant ) stays as an unrefined feature oil.
Afterwards, have the flask sit on the wire guaze on the iron ring and stand and attach it to the distillation