Enzyme Temperature Lab Report

Hypothesis: Very high temperature will denature and decrease the rate of enzyme reaction. Very low temperature will also decrease the rate of enzyme reaction. Procedure: In this experiment, 3 pieces of potatoes were put into 3 different test tubes of varying temperatures.

A simple change in temperature, a molecule out of place, and a sudden change in the pH level are just some of the things that can harm an enzyme 's reaction rate (the speed at which a chemical reaction proceeds) (5). To test the reaction rate of an enzyme, a lab was done to simulate what would happen to an enzyme under extreme conditions. The enzyme (represented by a hand) had to catalyze as many substrates as possible (represented by toothpicks) within 60 seconds. The experiment dealt with environmental factors such as extreme cold, presence of other molecules, etc. The lab that was simulated directly correlated to many of the topics discussed in class, like explaining the importance of enzymes and measuring the enzymes’ ability to function under different conditions.

The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.

The rateof reaction increases as the substrate concentration incteases until a certain point (Vmax) at which the reaction attains maximal velocity. Any Increase in substrate concentration after this point cause further increase in the rate of reaction because at Vmax enzyme moleclues are completed saturated with substrate molecules. 3.Effect of temperature At very low temperature enzymes are inactive. Enzymes activity increase gradually with the rse in temperature until a temperature at which the enzyme attains its maximal activity this temperature is called optimum temperature which lies between 37-40 c in humans.

1.Introduction: An enzyme is a large protein that acts as a biological catalyst which changes the rate of a reaction. It provides an active site which is an environment where a reaction can take place this is made up of amino acids. The structure and shape of the substrate, the structure and shape of an enzyme and the substance upon which the enzyme works all have to match exactly. This enables the substrate to bind, but it can 't do this if the shapes of the two are different. The Aim of Enzyme Catalase Experiment is making a series of experiments involving the enzyme Catalase which has been performed in order to determine some of the enzyme 's properties.

LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.

If more than 2 hours, it will lead to the star activity. Enzyme should be the last component which added to reaction before incubate it under 37°C. This is because the activity of the enzymes can be maintained at optimal temperature, 37°C. The mix components through pipetting the reaction mixture should be invert up and down slowly so that the mixture components are equalised. It can also follow by quick spinning in a micro centrifuge to ensure the components in the microtube are mixed equally.

LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.22.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3. Sucrase activity increases with increasing sucrose concentration Materials and Methods Effect of pH on Enzyme Activity 1. Dependent Variable amount of product (glucose and fructose) produced 2.

We added 1 ml of distilled water to test tubes labelled 2, 3,4 and 5. . To tube 1 we added 1ml of standard protein solution and recorded the concentration of the standard protein as 10mg/ml. To tube 2 we added 1ml of standard protein solution and shake it so that it can mix well. With a fresh pipette we removed 1ml from tube number 2 and added it to tube 3 then gently shake the tube. With another fresh pipette we removed 1ml from tube 3 and added it to tube 4, shake well.

Do the same for the other test tubes. Let the test tubes not be disturbed for about 3- 4 mins. Then add the Amylase solution to the Starch solution and start the stopwatch (immediately). After every 1 min take one drop from the test tubes and place then in the test plate that were

At last, at 95°C, or 85ºC, the enzyme activity is showed at being in its greater temperature. From black at 0 minutes, the color became bright yellow at 10 minutes, which specify the optimal temperature for

ABSTRACT: The purpose of the experiments for week 5 and week 6 support each other in the further understanding of enzyme reactions. During week 5, the effects of a substrate and enzyme concentration on enzyme reaction rate was observed. Week 6, the effects of temperature and inhibitor on a reaction rate were monitored. For testing the effects of concentrations, we needed to use the table that was used in week 3, Cells.

In the time it took for the enzyme to break up 0.23 grams of egg white at the temperature of 40 degrees, it broke down nearly three times as much in a temperature of 65 degrees. We can see from the graph that the hotter the water bath was the more egg white protein were broken down (because some of the test pieces gained mass, the graph shows the largest LOSS in mass in negatives to distinguish the difference between gaining and losing mass, so if the graph shows negative this indicates the largest loss of mass). Looking back at my hypothesis we can see that it was not correct although some of the predictions were more accurate than others. I predicted that the enzyme would work best in the two middle temperatures as they were the closest to core body temperature. looking at my results from the experiment we can clearly see that this was not true, but instead the two lower temperatures of 4 and 21 degrees Celsius showed a gain in mass while both higher temperatures of 40 and 65 degrees Celsius clearly indicate the breaking down of the particles as their is a clear decline of mass.

So the substrate can bind to enzyme easily. After the optimum temperature , the weak bonds holding the enzyme will break thus deactivating the active site. Changes in temperature may not only affect the shape of an enzyme but it may also change

A factor that could decrease an enzyme’s ability could be a shift in pH level or temperature. In our experiment, we tested whether the change in pH or the change in temperature affect the function of amylase, which is