Using Daphnia to Detect Water Safety
Due Date: Monday, the 31th of October, 2017
INTRODUCTION
Question: Can Daphnia be used to detect water pollution? How does the water type (source) affect the number of Daphnia and the accuracy and efficiency of testing water safety?
Purpose: The purpose of this project is to test different water sources for safety using the Daphnia species. This is important to the local people of Santa Barbara because it can be used to test whether the water & soil sources are safe from harmful pollution. Soil from various places in Santa Barbara will be collected and water from that soil, “run off”, will be tested against the Daphnia to see if it is toxic to them. Thus this experiment will find areas of soil in our
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When conditions become strenuous or stressful for the Daphnia to survive, death can begin to occur. Daphnia are ideal for this experiment because of their sensitivity to water quality. Units: Deaths per Water Type *Note - I will not be using this type of experimentation as is causes purposeful harm to the Daphnia, which kills more than they reproduce. This method was mainly an unproven theory that is included for informational purposes.
Water Toxicity Testing Method #2
To determine the safety, or toxicity of the water that the Daphnia are in, I have researched and found out how to find out whether the Daphnia are stressed, or if the water is bad for them. When the Daphnia get stressed due to a predator, they often die in great numbers. To prevent this from happening, I will get strained samples of the water that I am testing in order to eliminate the possibility of a larger predator that might cause false results. When the Daphnia cannot survive in the water conditions (contaminated) then the all of the Daphnia, which are female, will start producing males. Then the Daphnia after producing males, after 120 hours will start trying to reproduce sexually with the males instead of producing asexually in
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To measure the toxicity of water this way, one may use count the number of heartbeats per minute so that one can have a base reference to compare to. Once that is found, then one can take water that is from an outside source (ex: a school drinking fountain) and then move 20 of the Daphnia to that container. Then, one must wait 120 hours to see of the Daphnia heart beat aids lessened, and by how much. After that, the heartbeats found per minute can be divided into heartbeats per second and the two heartbeats from drinkable water to an unknown water source will be
On October 8th, 2015 our class took a field trip to the Conodoguinet Creek to test the water quality. We did 3 experiments to help us conclude the quality. During the first experiment we observed the different types of organisms in the creek. We also took tests for eutrophication in the water. Another experiment we performed were acidity tests.
A transfer pipette was used to place a single Daphnia magna into the well of a concave slide and any excess water was removed by using a Kimwipe tissue. The use of a coverslip was unnecessary so that the specimen isn’t crushed and killed. The D. magna had enough water to allow its gills to function but not so much that it could’ve actively swim. The specimen’s heart was located using a light microscope using the lowest magnification.
Upon completion of the experiments, Daphnia specimen exhibited extremely low heart rate while in its natural environment, which highlighted the negative effects of plant defense mechanisms on other
All organisms need oxygen to survive and when their is not enough oxygen, it leaves plants and animals to die. The area of water where there is a low count of oxygen are often called a dead zones. Also, an increase of the pH level in the water can be created because of algae blooms. The toxic killing algae can make humans sick. For the aquatic animals, these toxins attack mainly the organisms liver and nervous system (Nitrogen and
The experiment was done by observing the Himantura leopard and Dasyatis Americana interactions with a school of fishinside a 760,000 gallon tank at the Adventure Aquarium for 90 minutes. There were two Himantura leopard organisms and approximately five to six DasyatisAmericana. A school of fish was observed as three fishes that are approximately three inches away from each other. When either species of stingray came within five inches of the described school of fish, it was marked down if any of the fishes in the school separated from the school by swimming in an opposing direction of the other fishes of the school. If a fish separated from the school, it was marked down as “break apart” for the specie of stingray that caused the separation.
Villegas-Navaro, A., Rosas-L, E., & Reyes L., J. (2003). The heart of Daphnia magna: effects of four cardioactive drugs. Comparative Biochemistry and Physiology Part C: Toxicology and Pharmocology , 127-134. The purpose of this article was to test four cardioactive drugs (ouabain, verapamil, metaproterenol and metoprolol) to see what effects they have on the heart rate of Daphnia magna.
The purpose of this study is to understand the feeding rate of Daphnia magna. D. magna is a species of Daphnia that belongs to the suborder Cladocera (Ebert, 2005). They live in freshwater and feed on small, suspended particles in the surrounding water. They are filter feeders. Leaf-like appendages, called phyllopods provide a filtering apparatus for the collection of food.
The poisons such as DDT have the greatest affect on organisms at the top of food chains . for example , the white tailed eagle would have a greater concentration of DDT in its body than fish it feeds on . Top of the food chain large concentrations of DDT may kill organisms , decline its immune system , cause deformities , or damage its ability to reproduce . DDT can also weaken the shells of bird eggs. When eggs break too soon , bird embryos die .
Millions of people have faced with living-and-death issue due to constant use of contaminated water. Polluted water source causes the deleterious impact on the community. The common consequence is the rising rate of people at higher risk of acute and chronic diseases relating to water pollution such as dermatitis, digestive diseases, diarrhea, and cancer has risen. In several regions, patients diagnosed with cancer, gynecologic infections account for 40-50% of cases, due to polluted water sources. Polluted water and poor sanitation claim the lives of about 9,000 people per year in Vietnam, according to the Ministry of Health and the Ministry of Agriculture and Rural Development.
Data analysis and t-test statistics have supported the hypothesis that Daphnia heart rate would increase when exposed to tobacco extract. Furthermore, tobacco exposure had negatively affected the overall health of the Daphnia specimen. Its heart rate became unusually low, and even after the release to its culture it appeared to be motionless, and possibly died as a result of the experiment. Unfortunately, the concentration of the tobacco extract was unknown, and had it been lower, the effects could have been different. Nonetheless, the experiment was successful due to the supported hypothesis.
Corotto, F., Ceballos, D., Lee, A., & Vinson, L. (2010). Making the Most of the Daphnia Heart Rate Lab: Optimizing the Use of Ethanol, Nicotine & Caffeine. The American Biology Teacher , 176-179. The purpose of this article was to test the effects of chemicals on Daphnia magna.
Being able to raise and observe different species of marine life in a tank is good to protect a variety of marine species. One can observe how they act toward each other and many other interesting behaviors. This paper will be describing three topics, Beginning with Saltwater fish, The Arc - eye Hawkfish (parachirrhites arcatus) and Filefish. Eye Hawkfish and Filefish are two types of marine fish. There are certain things to consider when keeping fish in salt water tanks, to understand and start with the basics, here is what needs to be done first to have a saltwater aquarium.
Tenebrosa, factors caused by living things, known as biotic factors, also play a role in the distribution of P.Maculata. The first important biotic factor key in a P.maculata’s life is predators. As mentioned prior this organism is unable to move quickly in the water and like all organisms in the ocean is hunted as a means of food. As it cannot ‘outrun’ predators the organism, to deter predators, releases a unique toxin identified as tetrodotoxin. This toxin is extremely harmful and successfully enhances the survival of P.Maculata.
The 100% death may be reached 2-3 days after the infection with a dose of 107 – 108 cfu/ml, but 6-7 days with a dose of 105 – 106 cfu/ml (Triyaningsih et al., 2014). It is also known that A. hydrophila is frequently related to human diarrhea; as reported by Alberts et al. (1990), 12.2% of toddlers with acute diarrhea in Dhaka, Bangladesh, were positive to have A. hydrophila. Based on those facts, a quick monitoring system is needed to monitor the fluctuation of A. hydrophila concentration in fish cultivation ponds and also to make the quality control of drinking water source more
Aquaculture in India Aquaculture is the cultivation of the natural produce of water (such as fish or shellfish, algae and other aquatic plants). Mari culture is specifically marine aquaculture, and thus is a subset of aquaculture. Some examples of aquaculture include raising catfish and tilapia in freshwater ponds, growing cultured pearls, and farming salmon in net-pens set out in a bay. Fish farming is a common kind of aquaculture. India offers a huge potential for aquaculture development.