Determining the role of respiration and photosynthesis in spinach (spinacia oleracea) leaves under varying light conditions
Introduction:
Photosynthesis is the process of taking CO2, water and light energy and turning it into glucose and oxygen. The chemical formula is 6CO2 + 6H2O + light energy = C6H12O6 + 6O2 and this is a light dependent reaction which occurs in the chloroplast of plants. The glucose will be used in cellular respiration for energy and some of the oxygen will exit the plant through the stomata to provide the world with air to breath (Simms, 2017).
Cellular respiration is the next step which is a light independent reaction which takes the glucose and some oxygen from photosynthesis and produces CO2 and H2O. The balance of
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The first control variable was time and the time was kept at a constant throughout the three experiments so that there would be no flaws in data. If time would be a dependent variable the data would be different than that of which was collected. The second constant of the experiment was spinach, in each of the experiment’s there was 5 pieces of spinach that was put into the reservoir(probe). The last control variable for the three experiments was the placement of the probes, whether the probe was in the light or in the dark. (Simms, 2017).
Spinach leaves under white light should produce more O2 because photosynthesis is a light driven process.
The second experiment was White Light versus Blue Light which was conducted the same way as the first experiment. By putting 5 pieces of spinach into a probe and placed on a light in which it sat on the light for 600 seconds while the O2 and CO2 sensor read what the spinach was giving off. (Simms, 2017).
The wavelengths that are present in the blue light, are seen on a red through purple spectrum in which blue is found at 475 nm. These colors that are represented in wavelengths are shown as different colors. Each color has a specific number in nanometers in which they become visible to the human eye. (Simms,
+ ATP Although plants and animals have different methods of obtaining glucose, the cell respiration process occurs in both types of organisms. Many external factors in the environment may affect the organism's’ rate of respiration such as the temperature of the surrounding,
Both photosynthesis and aerobic respiration are necessary processes in plants and provide energy. Aerobic respiration uses glucose (sugar) and oxygen to form carbon dioxide and energy while photosynthesis uses carbon dioxide and water to form glucose and oxygen. Photosynthesis may only occur during the day time while aerobic respiration occurs all the time. Since an increase in carbon dioxide levels indicates an increase in aerobic respiration and a decrease in photosynthesis, we can measure the net exchange of carbon dioxide levels and determine whether more photosynthesis or aerobic respiration at a particular time has occurred.
Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.
In this particular experiment the constant condition, the factor that was never changed, was the room temperature water. The independent variable, the variable the student affected, was how much water was placed into the beakers. The dependent variable, a variable that was affected by the independent variable, was the time it took for the tablet to fully dissolve. The control group, which did not receive treatment from the students, was the 50 ml of water as a result of being the medium between the three measurements of water. Finally, the experimental group, the subject that was exposed to the variable during the experiment, was the 25 ml and 75 ml of
The first part of this lab was to get a chromatography, spinach and a quarter. The next step was to draw a line of the chromatography and rub the spinach leaf on it with the quarter. After this, the next step was to place the chromatography paper inside the tube and allow the solvent to rise to the paper. The final step was to remove the paper and mark the spots where the colors had shown up as they would disappear soon after. By doing this lab, it was possible to see all the different accessory pigments as well as the chlorophyll.
photosynthesis and cellular respiration are extremely important in the cycle of energy to sustain life simply because nutrients would not metabolize in a productive manner. Both have numerous stages in which the process of energy occurs, and relationships with organelles located in the eukaryotic cell. Photosynthesis is a process by which green plant and other organism manufacture their food using sunlight to synthesize foods from carbon dioxide and water while cellular respiration is the oxidation of organic compounds that occurs within cells, producing energy for cellular processes. Photosynthesis occurs within organelles called chloroplasts. These organelles can absorb light, and are located inside of leaves.
When the separation finished, I found different levels of colors. Which respectively top to bottom are Yellow-green, Blue-green, Yellow, Orange. We can Identify and label each pigment using the following information. Chlorophyll b Yellow- green Chlorophyll a Blue-green Xanthophylls Yellow Carotene Orange Figure1.
VARIABLES: There are independent variables, dependent variables, and controls. the one that is being tested and the one that is the inconsistent variable in the Independent variable like the volume and surface area of the agar cubes. The variable that is kept consistent is the dependent variable such as the percentage of diffusion of pigment in the agar cubes. The constant variable is the features of the experiment that is kept the same throughout the entire experiment such as, the amount of time the agar cubes are left in the beaker and the amount of acid in the beaker.
He Found this out by following on to Joseph Pristleys theory and procedure he put a small plant in a small transparent box with a lit candle then exposed the box to direct sunlight and waited a few days and when he came back and saw that the candle was still lit. Then he tried the experiment again but now in the dark and when he came back again a few days later he discovered that the candle was not lit. Pristley concluded that the plant does not produce air in the darkness and that it produced co2 and that light is essential for a plant to produce
Cellular respiration is a fundamental biological process that occurs in plants and animals and generates energy. Its chemical equation is 6O2+C6H12O66CO2+6H2O + ATP, the exact opposite of the second stage of photosynthesis (after the first stage during which sunlight is converted into Adenosine Triphosphate (ATP)). The circulatory system transports the glucose (C6H12O6) from the digestive system to the cells, the Oxygen (O2) from the alveoli to the cells, and Carbon Dioxide (CO2) from the cells to the alveoli. In the alveoli, O2 diffuses into capillaries close to alveoli and CO2 does the opposite. O2 and CO2 are transported into and out of the human body by the respiration system.
Secondly they take in carbon dioxide from the air around them. Thirdly they absorb sunlight from the sun above. With these three things plants are able to produce oxygen through photosynthesis. Humans and animals perform an opposite process. Humans and animals take oxygen into their lungs through breathing.
Then, tests are performed to determine if the products of aerobic and anaerobic respiration are present in the flasks. The citric acid cycle consists of a series of chemical reactions used by all aerobic organisms to release stored energy through the oxidation of acetyl-CoA derived from carbohydrates, fats, and proteins into carbon dioxide and chemical energy in the form of ATP (Biology). The tests detect the presence of carbon dioxide and ethanol. Carbon dioxide should be present irrespective of the type of respiration taking place, but ethanol is present only if fermentation has occurred. Another factor that can indicate whether fermentation occurred or cellular respiration occurred is the amount of glucose utilized during incubation.
Research Question: How does the presence of light impact the rate of transpiration in plants? Aim: The aim of this experiment was to investigate how the presence of light affects the rate of transpiration in plants. Hypothesis: As light intensity increases, the rate of transpiration (water uptake) in a plant increases.
In so doing the carbon atoms and their electrons are combined with oxygen forming a gas which is easily removed from both the cells and the organism. Plants use both processes, photosynthesis to capture the energy and respiration to use
Linh Dinh; Omar Cherif; Alexandra Olsson IB2G/IB2B Dr. Young Photosynthetic Pigments Lab Report December 1, 2014 Photosynthetic Pigments Lab The aim of this lab is to separate photosynthetic pigments by chromatography, a method used to separate the components of a mixture, and to measure each pigment’s Rf value. There are different types of chromatography, and the one which this lab focuses on is paper chromatography. The components of the mixture, in this case, spinach leaves mixed with acetone, are pipetted at one end of the chromatography paper and hung in a jar slightly above solvent vapor to create the reaction.