The Use of PCR and Gel Electrophoresis to Examine the Pitx1 Enhancer Region in the Stickleback Fish of Big Lake
Hypothesis:
Due to selective pressures in a freshwater environment, the stickleback fish in Big Lake will not have pelvic spines due to an insertion mutation in the Pitx1 enhancer region.
Rationale:
Since these stickleback fish are found in Big Lake, which is a freshwater lake that does not experience a marine environment, they will not have pelvic spines due to the specific selective pressures they experience. In Big Lake, the calcium levels is 14ueqv/L, which is much lower than the calcium levels found in a marine environment. This can result in less bone growth, and a lack of pelvic spine found in the stickleback fish. There
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In the stickleback fish found in Big Lake (seen in slot #4 and #5) are about 1400 base pairs long. By using PCR, we were successfully able to isolate the Pitx1 enhancer region in the marine control, and both stickleback fish in Big Lake. The sterile water used in the experiment was not contaminated, as seen in slot #6 of the gel electrophoresis, which means the results of the experiment should be accurate and without any contamination. There is a deletion in the Pitx1 enhancer region of the stickleback fish found in Big Lake, since the Pitx1 enhancer region is approximately 117 base pairs shorter than the marine …show more content…
In my hypothesis, I state that the stickleback fish would not have a pelvic spine caused by an insertion mutation in the Pitx1 enhancer region. According to the gel, the stickleback fish in Big Lake do not have the pelvic spine due to a deletion mutation found in the Pitx1 enhancer region. This can be further proved by the phenotype of the fish found in Big lake. The stickleback fish found in Big Lake (seen in figure 2) is smooth across the bottom, with no pelvic spine present. This proof supports the results of the gel
The design relied on two Schmitt triggers to generate the two different tones while using the transistors to act as a switch. This causes it to trigger continuously between two unstable states, allowing automatic switching between two frequencies producing two different tones. The RC values between the two Schmitt triggers will differ. Capacitors charge and discharge faster when it’s resistance is smaller.
Discussion 1. Zn0 (s)+ Cu2+S6+O42-(aq) →Cu0(s) + Zn2+S6+O42-(aq) Zn0(s) → Zn2+(aq) + 2e- Cu2+(aq) + 2e- → Cu0(s) Zn0(s) + Cu2+(aq) → Zn2+(aq) + Cu0(s) Oxidant (oxidizing agent) is the element which reduces in experiment.
%% Init % clear all; close all; Fs = 4e3; Time = 40; NumSamp = Time * Fs; load Hd; x1 = 3.5*ecg(2700). ' ; % gen synth ECG signal y1 = sgolayfilt(kron(ones(1,ceil(NumSamp/2700)+1),x1),0,21); % repeat for NumSamp length and smooth n = 1:Time*Fs '; del = round(2700*rand(1)); % pick a random offset mhb = y1(n + del) '; %construct the ecg signal from some offset t = 1/
1. The test subjects will prepare for sleep by acquiring everything needed for the subjects’ sleep preferences. 2. The test subjects will all set alarms on their smartphones for approximately 6, 8, and 10 hours after the subjects’ enter the resting period (Subjects may wake during the resting period for the bathroom, but they must not stay awake for more than ten minutes at a time to prevent as much deviation as possible.). 3.
1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.
Primitive fish, like lampreys and hagfish, have receptors that combine both water genes and air genes. This clearly shows that these primitive fish arose before the smelling genes split into two types. The number of odor genes have increased over time, from relatively few in jawless fish to the enormous number seen in mammals. The extra genes in mammals are all variations of the genes found in jawless fish. Therefore, the large number of odor genes in mammals arose by many rounds of duplication of the small number of genes in primitive organisms.
Genomic Recombination and Deletions in Acinetobactor baylyi ADP1 Shivani Patel Fall 2015 BIO 493 Introduction: Gene duplication and amplification is a process by which genetic diversity can be created and selected for. Through the understanding of gene duplication and amplification, scientists can garner insight on medical conditions associated with this phenomenon (Seaton et al. 2012). Not only can gene duplication and amplification increase genetic diversity, it can also increase the fitness of bacteria by allowing an increased production of essential nutrients or a gene to gain a new function (Dhar et al. 2014). However, gene amplification is not the only large genome change that can occur in organisms.
70 common limpets were identified in the 40m bracket identified as the littoral zone, between 0m to 40m. The common limpet employs a range of adaptations to survive the abiotic and biotic conditions associated with the littoral zone. Firstly, the limpet uses a structural adaptation to overcome the harsh wave action in the tidal zone, by using their radula to grip on to imperfections in the rock. Furthermore, they employ a behavioural adaptation of grinding their shells down into the rock, to further ensure they are not swept away by the tide. Additionally, the limpet utilises a behavioural adaptation regarding its tough shell and powerful radula, to defend against predators.
If the genetic aspects of the two populations show significant differences, then it is reasonable to make inference about their divergence. Johnson and Marten (1988) illustrated the differentiation of allopatric populations by examining 41 genetic loci in 11 breeding populations through electrophoresis. By examining the allelic frequency in coastal and interior populations, some alleles appearing in one population with high frequencies do not exist or have low
The Great White Shark The Great White Sharks, known mostly because of their white underbellies, are one of the most powerful aquatic animals in the world. They can swim at about 25 Miles Per Hour (40 Kilometers per hour) because of their strong muscles and forceful tails. In addition to that, male Great Whites can grow around 11.5 to 13.1 feet long, while females can grow from 14.8 to 16.4 feet long. This paper will demonstrate how Great White Sharks are an important part of their ecosystem, how their diets work and will adequately describe their habitats. As predators in their ecosystem, Great White sharks help maintain the coral reefs and seagrass habitats.
To determine if a fish is heterozygous or homozygous at the SFMSTR5 locus, the number of bands in a particular lane are analyzed. This gel is difficult to analyze due to the fact that it "smiles". When labeling the gel, the allele number is based on where the band is located between the 300 and 400 bp bands on the marker lane. Some of the alleles could be classified as either allele 1 or 2 due to the smiling effect. The classification of the alleles was done by comparing the band location to the location on the marker
In this experiment, a virtual program designed to demonstrate the swimming of a virtual fish, was used. This program is called SWIMMY. SWIMMY was used in this experiment to determine the circuits that are used in the movement of an animal. This is done by presenting the neurons and the neural circuits in a body which can allow and show the movement of the fish’s tail virtually. The movement of the fish tail occurs by the activation of motor neurons.
Vertebrates are known to be animals with backbones. Tooth reduction is one of the major evolutionary trends that developed among major vertebrate groups that allowed for the transition from aquatic to terrestrial life. Evolution of limbs and being able to breath air are other evolutionary trends that took placeThese trends include improved respiration and protective and insulating body coverings. More over the transition from water to land also included changing to more efficient reproductive methods like having a placenta for some animals or egg layers for other animals. Lastly, the morphology of organisms evolved such that for land they would have paired, muscular appendages used for crawling and
Lab 1 helps create a better understand of the changes in crystal structures when the annealing and quenching process is applied to 1020 and 1080 steel. The numbered steel refers to the ASTM grain-size number. Formula 1 is used to solve for the grain size. n=2^(G-1) Equation (1) at 100x magnification Crystal structures change shapes which changes the strength of the material and its properties. The metal might become soft, brittle, hard, or ductile.
Activity 1 Increasing extracellular K+ reduces the net diffusion of K+ out of the neuron through the K+ leak channels because it caused to decrease in the concentration gradient. Increasing extracellular K+ causes the membrane potential to change to a less negative value because extracellular K+ is increasing, which it will cause intracellular K+ to be less. A change in extracellular Na+ did not alter the membrane potential in the resting neuron because there are a lot of K+ leak channels than Na+ leak channels The relative permeability of the membrane to Na+ and K+ in a resting neuron is that Na+ leak channel is less, but K+ leak channels has more so the membrane become less permeable to Na+.