Lab # 1: Examining And Verifying Various Macromolecules

Name: Avishak Deb Roy Partners: Leevell Penn, Varugh, Butler Bio 101 Lab Report #1 02.22.2018 Swimming speed of paramecium tetraurelia in different levels of treatment. Introduction Paramecia is a unicellular Protista which are naturally found in aquatic habitats. It is easily cultured in the laboratory. It is oblong shaped and covered with short hairy structure called cilia. Paramecia does not pose any health or ethical concerns and the population can be maintained if there is a food source such as Enterobacter (Biological Foundation 7).

The presence of macromolecules is able to be detected in solutions such as glucose, sucrose, starch, and proteins as well as other common foods. These other common foods include oats, soda, gelatin, and apple juice. There are four classes of macromolecules such as monosaccharides, disaccharides, polysaccharides and proteins. Each of these can be found using different tests such as the Benedict’s test, the Iodine test, or the Biuret test. Although there is no specific test for disaccharides it can be determined if the original color has not changed.

This assay is a colorimetric assay based on an absorbance acid shift dye referred to as Coomasie Blue. The dye binds to the protein lysozyme if present in the solution. The more protein present the more dye more protein will become bound to it. A color change occurs from Brown to blue to indicate the presence of lysozyme. This was then read at an absorbance of 595nm to determine the concentration of each fraction

Negative: Water Constant: Iodine solution (indicator) Hypothesis: At least two out of the three procedures will be indicated as starch, due to the fact that amylase is the first enzyme that breaks down carbohydrates Materials: • Paper • Pen/pencil • Saliva • Water • Hydrochloric acid • Pipette • Iodine solution • Construction paper • Timer Procedure: 1. A sheet of paper was

In order to learn more about macromolecules, we performed an organic compound experiment. The purpose of this experiment is to find and test the presence of carbohydrates(Starch & Sugar) and proteins in different food using tested distilled water as indicators(independent variable). Food that will be tested in this experiment are cookies, milk, hot dog, pretzels, applesauce, cake mix, popcorn, and potato. My hypothesis is that if the food listed are tested for carbohydrates and proteins, then most of them with the exception of water, will be positive. Materials that will be used in this experiment are 8 regular test tubes, cookies, milk, hot dogs, pretzels, applesauce, cake mix, popcorns, potatoes, tap water, 500ml pyrex measuring cup, Benedict’s solution, Biuret solution, and Iodine solution.

In the lab, starch was mixed with ten drops of Benedict's and tested negative for sugar and the color changed to blue.

For instance, we could not conclude that mitochondrial activity is present in Supernatant II. However, our experiment showed that the boiled corn kernels did not undergo any mitochondrial activity while the raw corn kernels did. This might indicate that raising the temperature might have an effect on the function of dehydrogenase. Moreover, our found that starch granules are present in both sediment I and the “gunk”. Indeed, some parts of this experiment were not successful because the procedure was not followed

Starch solution is then placed into the test tube at a quantity of 5 mL. 5 drops of Lugol’s Iodine solution is added to the test tube. If the color changes, then it is known that starches are present in the solution. Proteins are next tested. In order to do this, 5 mL of gelatin solution is added to the test tube. 10 drops of Biuret’s reagent are added to test for protein.

Proteins are an essential part of all living organisms. Proteins are macromolecules made up of amino acid chains. These chains of amino acids are held together by peptide bonds to from polypeptide chains. Each proteins function is determined by its own unique three dimensional shape and active site. Proteins have multiple functions that are important to all cells.

The iodine test determines the presence of starch in biological materials. It is predicted that, if starch is not present, the solution with iodine remains yellow. However, if starch is present the solution with iodine becomes a blue-black colour. Plants have starch as the storage polysaccharide (glucose units held together by glycosidic bonds) while animals have the equivalent of glycogen. In this experiment, the dark blue colour is visible because of the helical amylose and amylopectin reacting with iodine (Travers et al., 2002).

Prior to the amylase extract being placed into the wells, the wells were prepared with 3 drops of iodine solution which works as an indicator of starch presence. We measured the presence of the starch with a 5 number color system. The lightest color, yellow, being number one and having the lowest concentration of starch, while the darkest color, a blue black,being number 5 and having the highest concentration of starch. The results showed that the optimal temperature for the bacterial amylase, Bacillus licheniformis, was 55℃, while the optimal temperature for the fungal amylase, Aspergillus oryzae, was of 25℃. The bacterial amylase, Bacillus licheniformis, had the

REGULATION OF GENE EXPRESSION AND THE DIFFERENCES BETWEEN EUKARYOTES AND PROKARYOTES IN THIS PROCESS Every day in our body and each cell there are a wide range of mechanisms that helps the cell to survive and also helps the organisms to survive as well. The part of these mechanisms is increasing and decreasing the production of specific gene products (either proteins or RNA), and is known as gene regulation. Moreover this process is happening in both eukaryotes and prokaryotes, but there are some differences between them. This essay would firstly, focus on the process of gene expression and control (regulation) of that, secondly the differences between the prokaryotes and eukaryotes in this in regulation of gene expression.

Uncontrolled Environmental conditions Atmospheric conditions The controlled variable Concentration of amylase was kept under control by measuring the amount of amylase used and also it was made sure the percentage of amylase used was 1%. The Amount of amylase/starch used were kept to 5cm3 at all times. Materials needed Beakers Bunsen burner Test tube Thermometer Stopwatch Test plate Glass rod Starch Amylase solution Water bath Iodine solution. Test tube holder Labels Marker Procedure First 5 test tubes were taken and labeled with numbers from 1 to

Biuret test is a test which is utilized to indicate unhydrolyzed proteins. When there are peptides in a solution, a copper (II) ion forms violet-coloured coordination complexes in an alkaline solution. The biuret test can be utilised to analyse the concentration of proteins due to peptide bonds that occur with the same frequency per amino acid inside the peptide. In this experiment, the colour changed to purple to indicate the presence of protein. The pH was found to be 7, which is in the range of a healthy person’s pH (which is 7.4).Benedict`s solution is made up of alkaline copper sulphate and sodium citrate (blue in colour) (Danson and et al, 1996).

Determining an effective assay is often difficult; but the more specific the assay, the more effective the purification. For enzymes, which are protein catalysts the assay is usually based on the reaction that the enzyme catalyzes in the cell. In this experiment, amylase enzyme is tested. it hydrolyzes starch to monosaccharides. The amylose component of starch complexes with iodine as follows and produces blue to purple complex.