From a worldwide public health prospective, reducing deaths from cervical and other HPV induced cancers is arguably the most important goal of an HPV vaccination program. Sustainable vaccination programs that protect as many women as possible from persistent infection by at least HPV16 and 18 would seem to be the most practical means of approaching this goal (Schiller and Haefliger 2006). Two prophylactic vaccinations against HPV have been licened by US Food and Drug admintration for use of young women ( Gardasil, a quadrivalent vaccine produced by Merk and approved in 2006 and Cervarix, a bivalent produced by Glaxo smith Kline and approve d in 2009). Both vaccines protect against HPV 16 and 18 and Gardasil also protects against HPV and 11 …show more content…
At each PCR cycle it is possible to measure the amount of amplified product. The detection is performed using non-specific fluorescent dyes that intercalate with any double-stranded DNA or using sequence-specific DNA probes. After each cycle, to estimate the DNA concentration, the fluorescence is measured with a detector and is compared with a control used as reference. Given its capacity to detect the presence and abundance of a specific DNA sequence, RT-PCR techniques have been developed to quantify HPV-DNA in clinical samples (Molijn et al. 2005) and (Dutra et al. …show more content…
Most laboratories use consensus primers targeting the L1 region, since it is the most conserved part of the genome, referring to the assay as L1 consensus PCR. Amplification of each of the primer sets will result in different size amplicons and consequently can result in a variation in sensitivity for detection of certain HPV types, particularly when samples contain multiple genotypes. There are numerous L1 consensus PCR primers that can be used (Morris 2005). Other example of consensus primers is the GP5/6, incorporating one forward and one reverse primer aimed at short regions of homology conserve amongst HPV types 1, 6, 8, 11, 13, 16, 18, 30, 31, 32 and 33 To improve efficiency, part of these sequences were used to elongate GP5 and GP6 at their 3’ ends to generate the primers GP5+/6+. The GP5+/6+ primer set generates a 150bp amplicon and reveales an improved HPV detection, reflected by a 10 to 100 fold higher sensitivity, compared with the GP5/6 (Dutra et al. 2012). The third option is to combine a number of distinct forward and reverse primers, aimed at the same position of the viral genome. These primers do not contain random degeneracies, but may contain inosine, which matches with any nucleotide. Using a defined mixture of non-degenerate primers has the advantage that the oligonucleotides can be synthesized with high reproducibility, and PCR is performed at
The STR length contrast is what is used to differentiate individuals. Gel electrophoresis then uses the STRs to create a DNA profile. The gel electrophoresis separates the STRs depending on their length and the pattern is then shown in fluorescent gel creating the profile. These profiles are then used by scientist to compare patterns between evidence and or suspects to determine a match or not a match.
The main way to find out if a patient has cervical cancer is to perform the Pap test. In the Pap test, a doctor will swap a sample of cells from the cervix and send it off to a laboratory for them to detect if there are any abnormalities. To confirm that the abnormalities are related to HPV, the doctor may then perform a DNA test of the Pap test sample. This DNA test is used to inform the doctor on whether the patient has a high-risk HPV which can cause cancer. This specific test is recommended for women who are at least 30 years old.
Controlling the spread of infectious diseases through immunization is one of medicine 's most significant accomplishments. Vaccination programs are proven to be a cost-effective means of disease prevention that have saved millions from death. Medical providers play an important role in the promotion of vaccinations; they can promote vaccination by following the standards for Adult Immunization Practice which include a four-step process: ♦ Assess immunization of all patients at every clinical encounter. ♦ Strongly recommend to patients the vaccines that they need.
HPV-16L1 had bound itself to the walls of cerebral blood vessels in both of the girls ' brains. The doctors that did the assessment also found that viral components of Gardasil that never should have crossed over into the brain, were found to be fully capable of doing precisely this.
Developments in HPV vaccines is important, not only for HPV prevention efforts, but also for the overall continued success of vaccination as one of public health 's most valuable
REF PCR results obtained from both normal and tumorous samples can then be compared to analyse differences in the mir-218 expression levels. Another method is
4. Molecular Diagnosis Parasite nucleic acids are identified by using the polymerase chain reaction (PCR). Even though this technique may be somewhat more susceptible than microscopy, it is of restricted effectiveness for the diagnosis of acutely ill patients in the standard healthcare setting. PCR results are often not obtainable rapidly enough to be of value in establishing the diagnosis of malaria infection. PCR is mostly beneficial for verifying the species of malarial parasite after the diagnosis has been recognized by either microscopy or RDT.
In Fred Sanger Method, the DNA to be sequenced serves as a template for DNA synthesis. A DNA primer is needed which is designed to be a starting point or the initiating point for DNA synthesis on the strand of the DNA to be sequenced. The primer is hydrogen bonded to the 3 ' end of the DNA to be sequenced. The DNA with the primer is divided into four separate reaction mixtures. Each reaction mixture contains all four dNTPs and in addition, one of the four dideoxy analogs (dideoxyribonucleoside triphosphates ddNTPs) of the deoxyribonucleoside triphosphates.
This makes it hard to know when you first became infected. Due to this rising threat and issue, scientists from Cleveland Clinics improvised a new medical innovation which may help women get rid of and be protected against the chance of developing cervical cancers. This is the self-administered HPV Tests. However, the Institute for Quality and Efficiency in Health Care ( IQWiG, Germany) analyzed studies that looked into the benefits of HPV tests in cervical cancer screening. They were particularly interested in whether HPV tests can help to detect major changes in mucous membrane cells (called high-grade dysplasia) earlier, whether this leads to an improvement in treatment and whether fewer women get cervical cancer and die as a result.
Investigating DNA Aim: To extract and examine DNA. Materials: As per page 40. Method: As per page 40.
Several factors contribute to the rejection of obligatory procedures of the HPV vaccine. Refusal influences include the sexually transmitted nature of HPV, concerns about the safety and efficacy of the new vaccine, and the perception that the vaccine manufacturer was overly involved in the policy process. Individuals argue HPV is not contracted through casual contact in the classroom setting, the vaccine diverges from other mandatory vaccines, and mandate supporters had difficulty debating denial of school entry contingent on proof of vaccination.
Vaccines are very controversial as some people fear that they cause the disease that they are trying to prevent. The vaccines must go through rigorous testing before distribution to the general public but the challenge with this is that not enough people are willing to offer their time for the trials, so they never get accurate results for the side effects. The task is to make safer vaccines just as effective but with less data: These consist of the current smallpox vaccines that cannot be given to immunocompromised people without causing harm; the tuberculosis vaccine, which is not suggested for HIV-positive infants; and the yellow-fever vaccine, which puts elderly people at risk of a yellow-fever-like illness. Surveillance on vaccines has
Finally, the amplified DNA regions are compare using a gel. DNA Profiling
The entire source for this population was all women attending this center over the last three years. All smears were examined in one laboratory used routinely by the center for the screening program, and all were examined by an experienced cytopathologist. 4.2.Sociodemographicand some other