Lab #1 Beer’s Law Stephanie Redmond Tuesdays 11 am – 1:50 pm Lab partner: Guilherme Bueno Dorea RESULTS Part One (Figure 1) (Figure 2) (Figure 3) (Figure 4) For part one of the lab, a Vernier SpectroVis Spectrometer was used to determine what type of olive oil was being analyzed. The grade of olive oil is determined by how the oil is extracted which also determines how much chlorophyll is left in the oil. These varying amounts of chlorophyll allow for the identification of the grade through spectrometry as the chlorophyll leaves distinct peaks in both the blue and yellow-red ranges. As shown in the figures above, different grades left peaks at different wavelengths and absorbencies. To determine the grade of the unknown …show more content…
Discussion The two most helpful features of the spectra to determine the unknown olive oil were wavelength and absorbancy. Also, the physical color of the olive oil was a very helpful indicator of the grade. Based on all of these facts, the unknown sample of oil was definitely extra virgin grade. It had both a dark yellow color similar to the extra virgin reference as well as a similar absorbancy peak. Based on the references take, a mixture of extra virgin and light olive oils would most likely have features resembling regular olive oil. It would have a lighter but still bright yellow color, and a wavelength and absorbancy between the ranges for extra virgin and light olive oil. Without the use of a spectrometer, the concentration of an unknown solution of nickel (II) nitrate could be estimated based on the coloring. The more concentrated the solution the darker green in appears and, vise versa, the less concentrated the lighter the solution will become as it is diluted by water. You could use Beer’s Law to calculate the unknown concentration of cobalt (II) nitrate as Beer’s Law states that a solutions concentration is proportional to the solutions concentration. Based on this, if you had the equation for Beer’s Law you could then plug in the unknown solutions absorbancy at 750 nm to find the
Coursework Equipment List • Boiling tubes (8) I will use these because this is where I will mix both the sodium carbonate and the strontium nitrate in order to form the precipitate. I need 8 because I am going to add 8 different amounts of strontium nitrate (1-8cm³) to the 8cm³of sodium carbonate. • Measuring cylinder (1) I will use this to measure the 8cm³ of sodium carbonate and the varying amounts of strontium nitrate to put into the test tubes. • Sodium Carbonate (enough to fill 8 boiling tubes with 8cm³/64cm³)
Terre Haute Brewing Company (THBC) is the second oldest microbrewery in the United States. The microbrewery was officially founded in 1837 and has gone through many transformations over the years. With them experiencing the prohibition of alcohol, the brewery had many challenges to face. The manager of Terre Haute Brewing Company said that their rich history is their biggest advantage. The microbrewery has old history that a company cannot just acquire.
B) Briefly describe each method, including the specific types of scientific tools and equipment used. Modified Mercalli Intensity Scale:
Since it is also a solvent, it has many cleaning uses. We predicted at once that is could be isopropanol because of its smell. Question: “What is the identity of unknown 6 in test tube 20?” Hypothesis: If I test the characteristic properties of unknown 6 then I will see its properties to be the same or similar to isopropanol.
This lab’s end result was to correctly identify each unknown solution using prior knowledge of chemical properties and the results of the first experiment conducted. Unknown solution D was the only colored solution, being blue while the others were clear. This made it easy to then match D up to Copper Sulfate because of its color. As unknown A and B were added together, lots of gaseous bubbles formed and revealed the fact that that reaction was the reaction between Hydrochloric Acid and Sodium Carbonate because it was the only reaction that produced a gas release. Unknown A and C produced the only yellow, brown precipitate just as the reaction between Sodium Carbonate and Silver Nitrate had previously.
Using two test tubes, label one “s” for substrate and the other “e” for enzyme. The substrate tube should contain 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL guaiacol and the enzyme tube should contain 6 mL of distilled water and 1.5 mL of peroxidase. Combine the materials of the substrate and enzyme tubes, mix the two using a clean transfer pipette, transfer a portion into a cuvette so that the cuvette is about half-full then cover the top of the cuvette with Parafilm and then place it in the spectrophotometer and record absorbance. Remove the cuvette and repeat recording absorbance at 1, 2, 3, and 4 minutes. Be sure to mix the cuvette and clean its surface with Kimwipes before each reading.
Mika Nijhawan 9/29- 10/10 2 MENTOS AND SODA LAB REPORT State the Problem Does the type of soda affect the size of the explosion, after mentos are added? Develop a Hypothesis If seven mentos to a 2L bottle of Diet Pepsi, then it will create the biggest geyser out of Coke, Fanta, & Root Beer because Diet Pepsi has potassium benzoate, aspartame, and the carbonation which react to mentos. Design an Experiment Materials List: 1 clear test tube 35 original flavored Mentos candies (2 1/2 rolls) 1 3x5 index card 4 2L bottles of soda (all different flavors/types) 1 2L bottle of soda water 1 100 mL graduated cylinder 1 400 mL beaker 1 pair of tweezers (preferably large) 1 small container Procedure: Stack seven mentos in the test tube When given permission take the test tube, soda bottles, index cards, and the remaining 25 mentos, to the designated area Place one of the four soda bottles on a flat area Open the soda bottle and place the 3x5 index card over it With the index card over the soda bottle, flip the test tube over and place it on the index card, so the test tube is lined with
3mL of the liquid in each of the vials were added into cuvettes and measured in the spectrophotometer. Before each time point the photo spectrometer was zeroed using a cuvette with 3mL of distilled water. If any of the results were considered unusual the machine was zeroed again and the sample was retested. The results from the spectrophotometer test were recorded in a table. The experiment was repeated six times to gain a sample size of six.
Record the amount of absorbance by converting transmittance every 5 minutes for a total of 20 minutes. Repeat all of these steps for the cantaloupe, banana, replacing the blank each time to recalibrate the spectrophotometer. After recording all the percent transmittance value, the data was then converted into absorbance value by using the absorbance conversion table. The information was placed on a plotted graph
The video “Important Advancements Because of Beer” and “The Poor Farmer Who Created Walmart” detailed the success of Beer and Walmart. Sam Walton was a successful entrepreneur, but his success did not come quickly. It took many years and even some failures before Walmart became the successful store that we know today. Sam Walton found a way to purchase goods for his new business cheaper than his competitors. This technique allowed him to sell the goods cheaper and ultimately beat his competitors in the marketplace.
If there is a color change, then it is known that protein is present in the solution. Finally, lipids are tested. 5 mL of water are added to 5 mL of oil. 5 drops of Sudan 3 are added, and if the color changes, then lipids are present. Next, the McMush is tested.
What You should know when using olive oil Olive oil is an oil obtained from the fruit of the olive tree (Olea European, in the family, Oleaceae), a type of tree, traditional belong Mediterranean. It is often used in cooking, cosmetics, pharmaceuticals, soaps, and fuel for traditional oil lamps. Olive oil is widely used, from around the world, but in particular, in countries, Mediterranean. Antiquity, olive oil, extracted from, manual methods, through, crushing and pressing of olives with stone millstones dedicated, to squeeze out the oil.
Joshua Miller 12/18/17 Fermentation Lab report Introduction The term fermentation refers to the chemical breakdown of a substance by bacteria, yeasts, or other microorganisms, typically involving effervescence and the giving off of heat (wikipedia). Sugars are converted to ethyl alcohol when fermentation happens. In this experiment we determined if yeast cells undergo fermentation when placed in a closed flask with no oxygen. Glucose and yeast are mixed together in a closed flask and allowed to incubate for about one hour.
This experiment aims to separate the components of the green colored food dye and get the TLC profile of each eluent collected. III. Experimental Procedure Before starting with the column chromatography for food dye, the right solvent must be chosen between 2-butanol with acetic acid, ammonia in butanol, 1 part 1-butanol 1 part acetic acid, and 2 parts methanol 1 part water. In choosing the appropriate solvent for column chromatography, the solvent system must give a TLC profile wherein most of the spots are well separated and has a Rf value within 0.3-0.5.
Conclusion The GC ethanol analysis method described above has a simple concept, its rapid, and extremely accurate, determining ethanol precisely without interference from other beverage components. With this method, it takes only 7 to 8 min to complete a sample analysis for the determination of ethanol content in a beverage sample. Analyst handling is minimized to prevent deviation in results or possible human error. This method requires a gas chromatograph and a digital integrator, both reasonably expensive and sophisticated pieces of equipment.