In the end, it was concluded that Unknown 30A may have a low molecular weight and was an amine because it turned the red litmus paper blue, after being soluble in water. Therefore, the solubility of the unknown occurred due to weak intermolecular attractive forces of hydrogen bonds. Small amines form hydrogen bonds in water. As a result, the litmus paper turned red to blue because the amine accepted protons from their bond with water molecules, and was basic. Functional group tests were performed on Unknown 30A to help identify and rule out functional groups present and not present. Chemical reactions carried out in functional group tests only work with certain functional groups. In functional group tests, control compounds are important …show more content…
The boiling point range of Unknown 30A was 65.0 °C-67.2 °C. The two degrees difference in the boiling point range indicated that there were very few impurities presented in the liquid. The theoretically possible identities of the unknown were primary amines that contained higher boiling points than the range because of the higher altitude lab. However, sec-butylamine, with a boiling point of 63 °C, was included as a possible identities because it was only two degrees off of the range. Therefore, it was too early on in the experiment to eliminate a potential identity that was very close to the range. Fourier transform infrared (FTIR) spectroscopy was obtained. FTIR spectroscopy represents transmittance of light, reflection, absorption, and emission indicated in gases, liquids, and solids. At specific wavelengths, infrared (IR) light—an interaction between light and matter— can distinguish types of energized bonds that absorb IR light. However, the bond and electromagnetic radiation frequency must match. Based on this lab, FTIR spectroscopy affirmed functional groups present in Unknown 30A because it revealed specific transmittance bands for those functional
The absorbance and the maximum wavelength of all eight standard solutions were determined using the same spectrophotometer in this section. First, approximately 3 mL of each solution was added into a cuvette using a plastic pipette. The solution was added until the level reached the frosty part of the cuvette and any bubbles were dislodged by gently tapping the cuvette against a hard surface. Then, a Kimwipe was used to clean the exterior of the cuvette. Once cleaned, the cuvette was transported by only holding the top edges.
Experiment 7 In this experiment we configured several DC circuits consisting of an emf and a network of resistors. The circuits were composed of a power supply, two DMMs, a circuit board, an SPST switch, and an assortment of known resistors along with one unknown resistor. We measured the current and voltage of the entire circuit as well as the potential drops across each resistor to determine the parameters of the circuit including the resistance, voltage, and current for each component.
1. A) Show that the relation R over bit strings where (x, y) is in R if and only bit strings x and y length 16 that agree on their last 4 bits is an equivalence relation. Define the equivalence classes and the partition induced by R. Answer: A relation R is said to be an equivalence relation if and only if it has all the following three properties: • Reflexive • Symmetric and • Transitive
I don't accept my current grade, because it is wrong, and my grade should be higher than D in anyhow according to Dr.Scandale's grading policy. The following explanation is made on my behalf. Lab 1.1 grade = 0 "what should be the correct grade?", and if different, explain why
Unknown Lab Report Abiola Oyewumi March 16, 2015 Unknown #16 Abstract An experiment was conducted to determine which of the following unknown bacteria was in test tube number 16: Escherichia coli, Enterobacter aerogenes, Klebsiella pneumoniae, and Salmonella typhimurium. Biochemical tests were used to help identify the unknown bacteria. The Citrate test, Urease test, Triple Sugar Iron Agar test, Voges-Proskauer test, and Methyl Red test were the biochemical tests used in this experiment.
The Unknown Identification Lab was an experiment that provided the opportunity to apply all the tests that were learned in the semester of lab, to identify the two bacterias that remain unknown. Gram- staining and two other tests will be used to identify the unknowns. This experiment is crucial to the understanding of each test, and can benefit in the ability to identify the characteristics of specific bacteria. Having a clearer understanding of the bacteria can further the research of bacteria for medicine, such as antibiotics. The understanding can also help the development of research in the environment.
Unknown Paper I Introduction This lab is a presentation of lab tests performed to finalize a conclusion based on results to identify the given unknown bacteria. The unknown bacteria was identified based on lab test results in the table provided in class for the possible unknown bacteria. The unknown bacteria identified as #36, and based on the lab tests is Enterobacter II Materials and Methods Catalase Test- this test determines whether bacteria have the enzyme catalase which catalyzes the breaks down hydrogen peroxide.
Use the evidence provided by the tests to identify the mystery powder. The mystery powder (#5) is the baking powder. My partners and I figured this out because the physical and chemical properties of both of the substances are very similar. For example, both powders are not soluble in water, and they both turned red when they reacted with the universal indicator.
In this lab, we tested 8 known ingredients to find what ingredients was in our unknown A and unknown B medications. We first tested the water solubility of our knowns and unknowns. We found that of the knowns, cornstarch and acetaminophen were the only ones not water soluble. The unknowns were also not water soluble. Th next test was the pH test.
Abstract: The purpose of this experiment was to identify given Unknown White Compound by conducting various test and learning how to use lab techniques. Tests that are used during this experiment were a flame test, ion test, pH test, and conductivity test. The results drawn from these tests confirmed the identity of the Unknown White Compound to be sodium acetate (NaC2H3O2) because there were no presence of ions and sodium has a strong persistent orange color. The compound then will be synthesized with the compounds Na2CO3 and HC2H3O2 to find percent yield.
Wavelength A graph was plotted on MS Excel with absorbance on the y-axis and wavelength (nm) on the x-axis. The absorption rate initially increased until the peak of 440 nm was reached (see Figure 1). After the decline of the first peak, the rate increased until the next peak was reached at 670 nm. The peak absorbance region was at 440 nm with an absorption rate of about .818 and at 670 nm with an absorption rate of about .431. Thus, the highest absorbance values were reached at the wavelengths 440 nm and 670 nm.
The possible explanations and changes to make are similar to the previous questions. Conclusion and Future Experiment 18. The identity of the product and unknown were 4-tert-butylbenzyl phenol ether and tert-butyl phenol respectively. The key to making this discovery was the melting point and TLC results!
Absorption spectroscopy is a tool most commonly used in analytical chemistry, however it is occasionally used in other fields such as molecular physics, atomic physics, and astronomical spectroscopy (“Absorption Spectroscopy”). Absorption spectroscopy is commonly used to quantify the amount of a substance present, which makes it very useful for determining how much of a certain product is present after a chemical reaction takes place (“Absorption Spectroscopy”). The absorption spectrum is the amount of radiation absorbed across a range of frequencies, it is mainly determined by the atomic or molecular composition of the specific material (“Absorption Spectroscopy”). The most common way to find the absorption spectrum is to direct a radiation beam at a sample and then detect the intensity of the radiation that passes through it, the energy that passes through can then be used to calculate the absorption of the substance (“Absorption Spectroscopy”). Absorption spectroscopy is very useful for analyzing chemicals because of how specific it is and its quantitative nature, due how to specific the absorption spectra is it is possible for a spectrophotometer to detect specific compounds within a mixture (“Absorption Spectroscopy”).
The other absorptions attributed to the molecule are shown in Table 5.4. Also the IR absorption peak can be compared as shown in Figure 5.3 and Figure 5.4. Table 5.4 Spectroscopic identification of erucic acid functional groups in FTIR Absorption frequency range(cm-1) Absorption peak (cm-1) Type of vibration 3020-3100 2920 =
The Raman spectroscopy allows the identication of homogeneous materials on the basis of their molecular vibrational spectra, obtained by excitation with visible laser light. This spectroscopy is based on the Raman ef- fect, which concerns to the molecular structure of the objects under analysis. When a monochromatic light impacts on a material, the light is scattered. Most of the scattered light has the same wavelength as the inci- dent light (the Rayleigh scattering) and a small portion is shifted in wavelength due to molecular vibrations and rotations (the Raman scattering) [8].