In this three-week long experiment conducted in the Bio 13 Lab, we were able to analyze a single nucleotide polymorphism (SNP) in our own genomic DNA and then determine our genotype at this specific SNP. In week one, we extracted genomic DNA from our cheek cells with swabs and prepared our DNA for PCR (Polymerase Chain Reaction) that would amplify the region with the intended SNP of interest. After one week and after the PCR was run outside of the lab section, the resulting PCR product was purified and treated with restriction enzyme Ahdl in order to prepare for the final analysis of our genotypes. In the third and final week of the project, we analyzed our PCR products by means of agarose gel electrophoresis. By the conclusion of the experiment, we had completed the analysis at the SNP of interest and determined our genotypes for this SNP. The first experiment involved in this four-week project was the extraction and …show more content…
Once the program was run, the machine began its thirty-five PCR cycles. Each step in these three step cycles is defined by a specific time span and temperature. However, before the first cycle begins, initial DNA denaturation occurred for five minutes at a temperature of 95 oC in order to ensure that all the DNA had become single-stranded. After this initial denaturation, the thirty-five PCR cycles could begin, first with thirty seconds of DNA denaturation at 95 oC, then followed by a drop of temperature to 58 oC for thirty seconds for the primers to anneal to its target sequence on the template DNA, and finally thirty seconds at 72 oC for primer extension in which DNA synthesis is carried out by Taq Polymerase. After the thirty-five cycles were complete, there was an additional five minutes at 72 oC for the final extension of the DNA to ensure that all synthesis had been completed. The completed PCR product is then held at 12 oC to preserve it until removed from the
For most sequences at position 4 and 5 we observe only the nucleotides G and T, respectively. There may be rare cases where other nucleotides may also be found. To consider such observations, we need to do a process called additive smoothing or Laplace smoothing to smooth the categorical data. [9] In this case, we add 4 sequences: AAAAAAAAA, CCCCCCCCC, GGGGGGGG, TTTTTTTTT.
V. EXPERIMENTAL SETUP & RESULTS The proposed dual T-NPC, dual PMSM topology and its modulation and control strategy are evaluated on an experimental setup as shown in Fig. 13. The experimental setup consists of two three-level T-NPC inverters feeding a dual three-phase 16 pole PMSM. The following capabilities of the proposed topology have been validated: 1) balancing DC-link voltages, 2) reduced output current distortion and 3) reducing capacitor RMS current.
1. The test subjects will prepare for sleep by acquiring everything needed for the subjects’ sleep preferences. 2. The test subjects will all set alarms on their smartphones for approximately 6, 8, and 10 hours after the subjects’ enter the resting period (Subjects may wake during the resting period for the bathroom, but they must not stay awake for more than ten minutes at a time to prevent as much deviation as possible.). 3.
1. There are two ways of maximizing points in this experiment. The first one is that I should connect myself to a vertex that is in the biggest component and purchases immunization. Since the probability of being infected is based off of expected value, I would have less than 1% chance of getting infected. The second way is that I try to make myself stay in the second-largest connected component.
1. What area/aspect of this setting is the most challenging? 2. In the setting, you work in, is there a certain population of patients you see more? How does this affect you?
3. Was there a particular DNA testing, the type of DNA or procedure that was used more often than others in the
1. This experiment was performed using cells from 3 different species, Vicia faba (broad bean), Allium cepa (onion), and Coregonus clupeiformis (whitefish), which obviously have variability between them. Onions are bulb plants, meaning they have a ball of stored nutrients underneath the soil out of which the roots protrude, where the broad bean does not have a bulb, having most of its mass above the soil. The whitefish is of course an animal, entirely different from the plants, including in how the cell cycle is performed. A cleavage furrow forms instead of a cell plate to perform cytokinesis, and centrosomes are present in its mitotic cycle, unlike in plants.
2.4 Band Division and Energy Computation: The power spectrum of the signal is multiplied by magnitude response of set of 33 triangular band pass filters and in the range 300Hz-2000Hz. Sub-bands are formed by using the logarithmic spacing. The positions of these filters are equally spaced along the Mel frequency, which is related to the common linear frequency f by following formula: Mel (f) = 1125* ln (1+f/700) (3) Mel frequency is proportional to the logarithm of linear frequency and which is close to the human perceptual system. 2.5 Sub Fingerprint Generation:
What is the term for the random arrangement of homologous pairs of chromosomes during the first division of meiosis? Independent Assortment 5. What role does the Polymerase Chain Reaction (PCR) play in producing a DNA Profile? PCR amplifies the regions of DNA with short tandem repeats and uses primers with fluorescent labels. This works by replicating the region of DNA several times.
\section{Facility Static and Dynamic Control}\label{Calibr} The facility calibration is the transfer function between the oscillating gauge pressure $P_C(t)$ in the chamber (described in ~\autoref{Sub31}) and the liquid flow rate $q(t)$ in the distributing channel, i.e. the test section. Due to practical difficulties in measuring $q(t)$ within the thin channel, and being the flow laminar, this transfer function was derived analytically and validated numerically as reported in ~\autoref{Sub32} and ~\autoref{Sub33}. \subsection{Pressure Chamber Response}\label{Sub31} Fig.\ref{fig:2a} shows three example of pressure signals $P_C(t)$, measured in the pneumatic chamber.
In this lab there were five different stations. For the first station we had to determine an unknown mass and the percent difference. To find the unknown mass we set up the equation Fleft*dleft = Fright*dright. We then substituted in the values (26.05 N * 41cm = 34cm * x N) and solved for Fright to get (320.5g). To determine the percent difference we used the formula Abs[((Value 1 - Value 2) / average of 1 & 2) * 100], substituted the values (Abs[((320.5 - 315.8) /
Most laboratories use consensus primers targeting the L1 region, since it is the most conserved part of the genome, referring to the assay as L1 consensus PCR. Amplification of each of the primer sets will result in different size amplicons and consequently can result in a variation in sensitivity for detection of certain HPV types, particularly when samples contain multiple genotypes. There are numerous L1 consensus PCR primers that can be used (Morris 2005). Other example of consensus primers is the GP5/6, incorporating one forward and one reverse primer aimed at short regions of homology conserve amongst HPV types 1, 6, 8, 11, 13, 16, 18, 30, 31, 32 and 33 To improve efficiency, part of these sequences were used to elongate GP5 and GP6 at their 3’ ends to generate the primers GP5+/6+. The GP5+/6+ primer set generates a 150bp amplicon and reveales an improved HPV detection, reflected by a 10 to 100 fold higher sensitivity, compared with the GP5/6 (Dutra et al. 2012).
The DNA gathered by the group bore positive results only on Test for Deoxyribose; compared to the standard solution, which bore positive results on all chemical tests, namely, Test for Deoxyribose, Test for Phosphate, Test for Purines, and test for Pyrimidines. Introduction Nucleic Acid is one of the essential biochemical molecules
DNA fingerprinting is a relatively new study, beginning in the 1980s, and revolutionizing forensic investigations. DNA fingerprinting refers to the identification of an individual based on the unique patterns found in their DNA samples. This was extremely new at the time and made identifying suspects more reliable than just going off of given alibis. DNA fingerprinting has caused some controversy in the effectiveness of the process, but since its discovery, many uses have been found such as, paternal identification, criminal cases in finding the suspect, and identification of the perpetrator in rape cases. Restriction Fragment Length Polymorphism (RFLP) is a method used for DNA fingerprinting by means of gel electrophoresis.
Finally, the amplified DNA regions are compare using a gel. DNA Profiling