In this experiment, 9-fluorenone, a ketone, was reduced to fluorenol, an alcohol. The product was then identified using melting point and IR data, and percent yield was calculated. Reduction is one of the two processes that occur during a redox reaction, and it involves the gain of an electron by one of the species. The other species in the reaction loses an electron, and is by definition oxidized. In this experiment, fluorenone, the oxidizing agent, was reduced, and sodium borohydride, the reducing agent, was oxidized. In this experiment, hydride reducing agents were used, since hydrides have spare electrons that they can donate to other compounds. Two popular hydride reducing agents, lithium aluminum hydride and sodium borohydride, were considered for this experiment. Since hydride reducing agents were used in this reaction, the reaction would have been extremely sensitive to proton sources, since …show more content…
This would be an excellent yield if it all indeed consisted of fluorenol, but given that -OH peaks were observed in the IR, and that a good yield for this reaction was around 60%, it is possible that this percent is artificially high, and that some of this yield consisted of impurities like water and methanol that had not evaporated away. Of the product that was lost, some was lost due to bad filtration technique, as some of the product was observed to have passed through the filter paper, and into the flask. Some of the product may have also clung to the vial, as the precipitate was difficult to remove from the vial in its entirety. In order to improve this yield, more care could have been taken while removing product from the vial, and during filtration, ensuring that the filter paper was sufficiently wet and no product passed through. As some error most likely occurred due to impurities, inflating the percent yield, the product could have been allowed to dry
Upon removing the fluorenone from the watch glass and re-weighing, a much more reasonable yield of 52 mg or 52% recovery was obtained. This discrepancy in weighing led to
1. Identify the range of senses involved in communication • Sight (visual communication), Touch (tactile communication), Taste, Hearing (auditory communication), Smell (olfactory communication) 2. Identify the limited range of wavelengths and named parts of the electromagnetic spectrum detected by humans and compare this range with those of THREE other named vertebrates and TWO named invertebrates. Figure 1: the electromagnetic spectrum source: www.ces.fau.edu Vertebrates Human Japanese Dace Fish Rattlesnake Zebra Finch Part of electromagnetic spectrum detected ROYGBV (visible light) detected by light sensitive cells in the eye called rods and cones.
The last goal was to determine the percent yield of a product formed during a reaction with the unknown compound. Experimental Design The first day of lab consisted of various preliminary tests that helped identify the unknown compound.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
The goal of the experiment is to synthesize a bromohexane compound from 1-hexene and HBr(aq) under reflux conditions and use the silver nitrate and sodium iodide tests to determine if the product is a primary or secondary hydrocarbon. The heterogeneous reaction mixture contains 1-hexene, 48% HBr(aq), and tetrabutylammonium bromide and was heated to under reflux conditions. Heating under reflux means that the reaction mixture is heated at its boiling point so that the reaction can proceed at a faster rate. The attached reflux condenser allows volatile substances to return to the reaction flask so that no material is lost. Since alkenes are immiscible with concentrated HBr, tetrabutylammonium bromide is used as a phase-transfer catalyst.
Absorption Absorption of chlorpyrifos varies with species to species. In humans, about 70% was absorbed after oral exposure of volunteers. For the metabolite, 3, 5, 6-trichloro-2-pyridinol (TCPY), the minimal dermal absorption was 1-3%. It is to be noted that chlorpyrifos (cpf) is rapidly absorbed and transported to the brain through oral dosing [66]. Distribution
Radioactively Labeled Azole Import by M. oryzae Radioactively labeled FLC (3H-FLC), (481 GBa/mmol, 13 Ci/mmol, 1 µCi/µL; 77 µM FLC) was custom synthesized by Amersham Biosciences. The drug concentration used during the import assay was well below the Minimum Inhibitory Concentration (MIC) for the strain (M. oryzae FLC MIC >32 µg/ml). To directly measure azole intracellular accumulation in the fungal cell, we used 3H-FLC in our drug uptake assay designed for M. oryzae.
Furthermore, the melting point of the experiment was obtained to be 151-154 ºC, which included the theoretical melting point of 153 ºC between the experimental melting point range. Introduction: Redox reactions are very important in organic chemistry and they involve transfer of electrons from one molecule to another. Oxidation occurs when a carbon atom becomes bonded to a more electronegative atom, causing the electron density on carbon atom to decrease (McMurray, 2012). That was the part that our experiment focused.
Tn 4351 was originally isolated from bacteroides fragilis [30] . The transposon was successfully introduced into Cytophaga succinicans, Flavobacterium meningosepticum, Flexibacter canadiansis, Flexibacter strain SFI and Sporocytophaga myxococcoides by conjugation [25]. Tn 4351carries two antibiotic resistance gene. One of the codes for resistance to erythromycin and clindamycin which is expressed in bactroides but not in E.Coli. The other gene codes for resistance in tetracycline and is expressed in aerobically grpwn E. coli, but not in anaerobically grpwn E. coli or in bacteroides.
The purpose of this experiment was to learn about metal hydride reduction reactions. Therefore, the sodium borohydride reduction of the ketone, 9-fluorenone was performed to yield the secondary alcohol, 9-fluorenol. Reduction of an organic molecule usually corresponds to decreasing its oxygen content or increasing its hydrogen content. In order to achieve such a chemical change, sodium borohydride (NaBH4) is used as a reducing agent. There are other metal hydrides used in the reduction of carbonyl groups such as lithium aluminum hydride (LiAlH4).
Unknown Lab Report Mikee Lianne Gonzales Biol 351- 1005 Holly Martin Unknown: # 76 Abstract This report is about identifying the respective genus of the given unknown organism. The goal is to show and prove the student’s understanding of microbiology and laboratory learned experimental techniques.
3. In this experiment, the percent yield was 90%. This number implies that there was little error in this experiment. However, this result could have been caused by certain external factors.
INTRODUCTION A gas chromatograph (GC) can be utilized to analyze the contents of a sample quantitatively or in certain circumstances also qualitatively. In the case of preparative chromatography, a pure compound can be extracted from a mixture. The principle of gas chromatography can be explained as following: A micro syringe is used to inject a known volume of vaporous or liquid analyte into the head or entrance of a column whereby a stream of an inert gas acts a carrier (mobile phase). The column acts as a separator of individual or chemically similar components.
This was proved by utilizing the IR spectrum to verify the C =O was not in the final product as it lacked the 1640 cm-1 peak. The melting point of 113-115 degrees C proved that the final product obtained was the E-Stilbene. The TLC plate proved that the E and the Z product was produced, show cased by the double intensity of the DCM spot to the final product’s spot, both which had an Rf of 0.92. The double intensity proved that both products were produced, but through heating and filtering, the Z-Stilbene was
Not having a high yield was probably due to poor technique that failed to extract a solution that is clear. There may have been contamination with NaSO4 because a pipette filter was not used. In the future, the drying agent should be filtered by a pipette filter rather than extracting the organic layer directly with a