1. Introduction Plants are the main source of food and medicine of humans since the times of ancient throughout the world. Many synthetic antioxidants such as butylated hydroxyl anisole (BHA) and butylated hydroxyl toluene (BHT) are known to have side effects causing liver damage. The expensive treatments, adverse effects of several allopathic drugs and development of resistance to existing drugs have forced us to look back into nature as a resource for therapeutics to a wide variety of human ailments. Therefore there is a need for isolation and characterization of natural antioxidant having fewer side effects which can be used in foods or medicinal materials to replace damage causing synthetic antioxidants [20]. A very important medicinal …show more content…
Estimation of total Flavonoid content by AlCl3 reagent method The total flavonoid content of methanol extract of leaves of A.malabarica was determined by the AlCl3 reagent method [4]. The extract (500 µg/mL) was mixed with 0.5 mL of 5% NaNO2 solution and allowed to stand for 5 mins. Then 0.3 mL of 10% AlCl3 solution was added and the mixture was allowed to stand for further 5 min. Finally, 1 mL of 1 M NaOH solution was added, and the final volume of the mixture was brought to 5 mL with distilled water. The mixture was incubated for 15 mins at room temperature and absorbance was measured at 510 nm. The total flavonoid content was expressed as quercetin equivalent (µg/mg of extract), which is a common reference standard. 2.4. DPPH radical scavenging assay The DPPH radical scavenging activity of methanol extract of leaves of Anisomeles malabarica was carried out according to the method [1].One mL of Plant extract of various concentrations (50-300 μg/mL) were mixed with 1 mL of 0.1 mM of DPPH solution in methanol. The reaction mixture was kept at room temperature for 30 min. Absorbance was read at 517 nm in spectrophotometer. The percentage of the radical scavenging activity was calculated as follows. % of inhibition = Control – Sample ×
For this lab, zeolite and magnetized zeolite were synthesized and compared with charcoal to find out with would be the most effective in the sequestering of Procion Red dye. Finding the concentration and absorbance of each zeolite, magnetized zeolite, and charcoal, along with a calibration curve, the best adsorbent is determined. Charcoal was the overall best sequestration of the Procion Red dye, since the adsorbent was highest compared to the others. Introduction Pollution has increased in the environment over the years, so the purpose of this experiment is to find the best adsorbent of chemicals to reduce the pollution.
Unknown Lab Report Unknown # 25 By: Jenna Riordan March 19, 2018 Bio 2843 1. Introduction Microbiology is the study of microorganisms found in all different environments throughout Earth, from the hot thermal vents at the bottom of the ocean to the ice at the top of a mountain.
ABSTRACT To catalyze a reaction, an enzyme will grab on (bind) to one or more reactant molecules. In this experiment we examined how increasing the volume of the extract added to the reaction would affect the rate of the reaction. The enzyme used was horseradish peroxidase which helps catalyze hydrogen peroxide. Using different pH levels, the absorbance rate of the reaction was measured to see at which condition the enzyme worked best. The rates of absorption were calculated using a spectrophotometer in 20 second intervals up to 120 seconds.
Abstract The purpose of this experiment is to test for mitochondrial activity by isolating different organelles using the differential centrifugation process. Studying mitochondria is extremely important because they control the death and life of the cell by regulating the apoptotic signals (Frezza et al 2007). Also they are responsible for the metabolic reactions (aerobic respiration) and the production of ATP (Frezza et al 2007). Three hypotheses were formed based on my knowledge.
There are few vegetables and fruits that turns to the color brown if their surface is exposed to oxygen. Once the veggies or fruits been exposed to oxygen, then the browning begins to appear, and electrons and hydrogen will be removed. This happens because of an enzyme called catechol oxidase. The enzyme will act on its substrate catechol to form a yellow compound which then reacts with the oxygen in the air and change into benzoquinone. The more concentration of the enzyme, the more browning appears.
The enzyme of turnip peroxidase is added in the equation to catalyze the oxidation. Objectives The objective
The effect of pH on the speed of enzyme interaction with substrate chemicals Hypothesis: About pH: If the pH level is less than 5, then the speed of the enzyme reaction will be slower. About temperature: If the temperature stays the same, then the speed of the enzyme reaction will not be completely affected. Background information: The function of enzymes is to speed up the biochemical reaction by lowering the activation energy, they do this by colliding with the substrate.
LABORATORY REPORT Activity: Enzyme Activity Name: Natalie Banc Instructor: Elizabeth Kraske Date: 09.26.2016 Predictions 1. Sucrase will have the greatest activity at pH 6 2. Sucrase will have the greatest activity at 50 °C (122 °F) 3.
Methods of Data Collection Measuring the independent variable: The pH (the independent variable) is being tested on the turnip peroxidase to observe the reaction rates. 5 levels of pH are required for these series of reactions so pH buffers of 3, 5, 7, 9, and 11 are to be placed in each of the waters that will be put into the cuvettes for the experiment. Measuring the dependent variable: A colorimeter must be used in order to calculate the reaction rate/absorbance level of the turnip peroxidase when the different pH levels affect it. The colorimeter can be used to measure the transfer of heat to or from an object.
These red colored pigments have drawn attention recently among nutritionists since it has powerful antioxidant. It’s due to its ability to quench singlet oxygen and prevent oxidative damage
Introduction Carotenoids are pigments that are found mainly in chloroplasts of plants and in some photosynthetic organisms. They are synthesised naturally in plants in order to absorb light during photosynthesis and protect plants against photosensitization. They are found in our diet, through eating fruit and vegetables and many serve as an important source of vitamin A in our diets, such as ß-carotene. The structure of the molecule determines the colour, photochemical properties, vitamin activity and its chemical reactivity on oxidising agents and free radicals.
No matter how much you tried to avoid them, almost everyone battles an ant infestation at one point or another. Its easy for them to get inside your home, since they are tiny and can fit in almost any crack within your home. Plus, they are attracted to the smells of food inside, and anything left out only makes the infestation worse. Here are a few remedies to get rid of ants. Borax & Powdered Sugar: Simply mix one part borax with 3 parts powdered sugar together in a small container.
pruriens seed extract and FeMPn were characterized with FTIR to determine the biomolecules contained in the extract that involved in the reaction to form FeMPn. The FTIR spectra of the extract and FeMPn are shown in Fig. 5. The FTIR spectrum of the extract showed a broad absorption band in an absorbance area of 3384.8 cm-1 that assigned to the overlapping of O-H stretching vibration of flavonoids, alkaloids, polyphenols, alcohols or water and N-H stretching vibration of amine compounds, due to the hydrogen bonding. The absorption band at 1627.8 cm-1 referred to C=C stretching vibration which is possible to be derived from aromatic ring in amino acid, while the absorption band in 1529.4 cm-1 referred to N-H bending vibration of amine which is possible to be derived from the L-dopa.
It is because of the arrangement of the hydroxyl groups that is located on the B ring affects ROS scavenging properties. The hydroxyl groups on the B ring donates hydrogen electrons to the free radicals which results in their
Abstract The unknown concentration of benzoic acid used when titrated with standardized 0.1031M NaOH and the solubility was calculated at two different temperatures (20◦C and 30◦C). With the aid of the Van’t Hoff equation, the enthalpy of solution of benzoic acid at those temperatures was determined as 10.82 KJ. This compares well with the value of 10.27KJ found in the literature.