Spinach Leaf Chromatography Lab Report

During this experiment, mitochondria were isolated from 20.2 grams of cauliflower using extraction buffer, filtration through Miracloth, and centrifusion. Twelve samples containing various volumes of mitochondrial suspension, assay buffer, DCIP, sodium azide, and citric acid cycle intermediates were prepared to be read by a spectrophotometer. The inclusion of the dye DCIP allowed for the absorbance of the reactions between the mitochondrial suspension and the TCA cycle intermediates succinate, malonate, and oxalate to be measured, as DCIP turns from blue to colorless as the activity of succinate dehydrogenase increases. Experimental Findings Increasing the number of mitochondria in the reaction did increase the reduction of DCIP relative to the amount of mitochondrial suspension present.

The bands of color travel the greatest distance are more soluble than the bands that traveled less distance. In this experiment, we did not see chlorophyll a or carotenoids pigments. This could be due to the fact that we used coleus leaves and grass leaves, which do not have a large quantity of carotenoids or chlorophyll a. If we were to use spinach, then we would have seen a different concentration of pigments because the spinach leaf contains certain pigments at a higher

This experiment is set up the study the effect of different sodium bicarbonate concentrations on the rate of photosynthesis in spinach (Spinacia oleracea). Photosynthesis is the process by which plants and other photoautotrophs synthesize organic compounds from carbon dioxide (Faculty of Science and Horticulture, 2018). Photosynthesis takes place in the chloroplast of a plant cell, where sunlight, water, and carbon dioxide are used in a reaction to produce oxygen and sugar (Reese 2017). In the photosynthesis reaction carbon dioxide is reduced to make sugar and water is oxidized to make oxygen. In this experiment oxygen production is being used to measure the rate of photosynthesis.

Using two test tubes, label one “s” for substrate and the other “e” for enzyme. The substrate tube should contain 7 mL of distilled water, 0.3 mL of hydrogen peroxide, and 0.2 mL guaiacol and the enzyme tube should contain 6 mL of distilled water and 1.5 mL of peroxidase. Combine the materials of the substrate and enzyme tubes, mix the two using a clean transfer pipette, transfer a portion into a cuvette so that the cuvette is about half-full then cover the top of the cuvette with Parafilm and then place it in the spectrophotometer and record absorbance. Remove the cuvette and repeat recording absorbance at 1, 2, 3, and 4 minutes. Be sure to mix the cuvette and clean its surface with Kimwipes before each reading.

The minor pigments play a secondary role in photosynthesis and transfer the energy the minor pigments receive back to chlorophyll for photosynthesis (Fry, 2014). If the spinach leaves’ pigments are separated by chromatography, then the pigments chlorophyll a, chlorophyll b, beta-carotene, and xanthophylls will appear on the chromatography

Quinn Nguyen Floating Leaf Disk Photosynthesis Lab Conclusion: Graph your results for all 3 trials on one graph. Label the graph, both axes, and provide a legend to distinguish each trial. What was the rate of photosynthesis for each variable? How many leaves floated per minute? Rate of photosynthesis (leaves/min) Spinach: (2-0)/25 = 0.08

Purpose: The purpose of this experiment is to see how long it takes for the 10 spinach leaf discs to undergo photosynthesis and thereby rise in the two solutions. Hypothesis: All of the leaf discs in the sodium bicarbonate solution should be floating before the discs in plain water because the bicarbonate is a carbon source that will allow photosynthesis to continue. Background: Light is absorbed by leaf pigments (chlorophyll) which makes electrons within a photosystem moved to a higher energy level.

Because carbon dioxide is absorbed by the plant during photosynthesis less carbon dioxide present in the chamber is a sign that photosynthesis is working. The four lights used for this experiment range across the light spectrum on both sides in order to test a wider variety of wavelengths. All lights will be placed directly on the spinach leaf at the same distance so as not to give any spinach leaf a different light intensity, which could affect the data. This experiment will be able to show which light, ranging across the light spectrum, will allow the Spinach to perform photosynthesis more efficiently.

Determining the role of respiration and photosynthesis in spinach (spinacia oleracea) leaves under varying light conditions Introduction: Photosynthesis is the process of taking CO2, water and light energy and turning it into glucose and oxygen. The chemical formula is 6CO2 + 6H2O + light energy = C6H12O6 + 6O2 and this is a light dependent reaction which occurs in the chloroplast of plants.

LABORATORY REPORT EXERCISE #5 INTRODUCTION TO THE COMPOUND LIGHT MICROSCOPE, PLANT AND ANIMAL CELLS Name_______________________________Section_____Teacher______________Date________ PRE-LAB QUESTIONS - answer the following questions using your textbook and valid internet sources. Be sure to cite your sources at the end of the prelab. You can type your answers to all questions except #1 and #9 directly into this document and then submit via Canvas. Type the answers for #1 and #9 at the end of the document. 1.

How did different colors of light affect c02 consumption in spinach leaves? Background Photosynthesis is a process by which plants and other autotrophs make their own food using sunlight energy. Plants take in water, carbon dioxide, and use sunlight energy to create glucose and oxygen. Plants use the glucose as energy and release the oxygen as a by-product.

The purpose of the Spinach Leaf Chromatography Lab was to determine how much chlorophyll a, b and carotenes and xanthophylls the Spinach leaf contained through measuring the distance traveled by the pigments. The hypothesis the group created was that a spinach leaf contained multiple pigments. The group after doing the chromatography of the spinach leaf then determined the rate or flow of migration using Rf(Distance pigment traveled/ distances solvent 2. Materials and Methods A 2x15 strip of chromatography paper was cut so that it would fit inside the test tube. Then a point was cut in the bottom 0.5 cm of the strip.

The 3 concentrations of enzymes were 0.5 ml, 1.0 ml, and 2.0 ml of turnip extract, while the substrate consisted of 0.1ml, 0.2 ml, and 0.4 ml of hydrogen peroxide. In a separate tube, the control was made up of turnip extract and guaiacol, known as the color reagent. This was recorded the absorbance every 20 seconds for 3 minutes.

Column chromatography set-up After setting up the column, 2 10-ml of the chosen solvent was obtained and was placed in two separate test tubes. Using a dropper, ~0.5 mL of the food dye was put into the column by dropping it at the side of the column in a circular motion. The chosen solvent was then added just after the green food

The hormone cytokinin causes this after the exposure of light. Chloroplasts contain chlorophyll and due to this the plant turns green. 1.1 Research question For this experiment, I want to find out what will happen to cress seeds that are etiolising (growing in the dark) when they are then exposed to light. To conduct this experiment, I came up with the following research question: ‘Will exposure to light after a set period of etiolation cause a plant to de-etiolize?’ 2.